To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药陈旧性腰椎间盘突出症(LDH)的近果并讨其理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检活性，原位末端标记(TUNEL)方法检凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶联检法30分钟。种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的与裂解的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转成红色合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)期效果并讨其治疗。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物成熟睾丸和精子中，为精子运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成量与裂解细胞数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH细胞活性，原标记(TUNEL)方法细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶法30分钟量。这种酶分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作LDH患的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和子，子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

MTT、LDH测细胞活性，原位末端标记(TUNEL)方法测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢存在于培养物上清中,可测法30分钟测量。这分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含也增高。