To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特酸脱氢酶特地存在于鸟类和哺类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性酸脱氢酶特异地存在于鸟类和哺类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成臜化合物。颜形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙相关肽和促生长激神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸存在于培养物上清中,可用联检测法30分钟测量。这种联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉组比较，双香豆能显著增强阿霉所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳氢酶特异地存在于鸟类和哺乳类动物的熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑（INT）红色甲臜合物。颜色形的量与裂解细胞的数目正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙相关肽和促生长激神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸存在于培养物上清中,可用联检测法30分钟测量。这种联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉组比较，双香豆能显著增强阿霉所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药陈旧性腰椎间盘突出症(LDH)的近远并讨机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟乳动物的成熟睾丸精子中，为精子的运动存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应MTT、LDH检测细胞活性，原位末端(TUNEL)法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽以作为LDH患者疼痛的神经化学指。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢存在于培养物上清中,联检测法30分钟测量。这种联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。