Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受的方法，并探讨小鼠附睾子在显微注射进入卵母细胞后的受。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮细胞在卵母细胞卵黄形成期，它的RNA和DNA的合成均较快，因认为滤泡上皮细胞合成分泌了一些物质参与卵黄蛋白的形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结合效率的体细胞与卵母细胞电合时可通过增加PVS中体细胞的数目来提高合效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母细胞又分裂成两个大小不对等的新细胞：一个相对很大的卵子（成熟卵细胞）和一个微小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 的:建立显的方法，并探讨小鼠附睾子在显注射进入卵母细胞后的能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮细胞在卵母细胞卵黄形成期，它的RNA和DNA的合成均较快，因认为滤泡上皮细胞合成分泌了一些物质参与卵黄蛋白的形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体细胞与卵母细胞电融合时可通过增加PVS中体细胞的提高融合效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母细胞又分裂成两个大小不对等的新细胞：一个相对很大的卵子（成熟卵细胞）和一个小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目:建立显微受精方法，并探讨小鼠附睾精子在显微注射进入卵母细后受精能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮细在卵母细卵黄形期，RNADNA均较快，因认为滤泡上皮细分泌了一些物质参与卵黄蛋白形。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融效率体细与卵母细电融时可通过增加PVS中体细数目来提高融效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母细又分裂两个大小不对等新细：一个相对很大卵子（熟卵细）一个微小次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 ： :建立显微受精方法，并探讨小鼠附睾精子在显微注射进入卵母细胞后受精能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮细胞在卵母细胞卵黄形成期，它RNA和DNA成均较快，因认为滤泡上皮细胞成分泌了一些物质参与卵黄蛋白形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融体细胞与卵母细胞电融时可通过增加PVS中体细胞数来提高融。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母细胞又分裂成两个大小不对等新细胞：一个相对很大卵子（成熟卵细胞）和一个微小次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的法，探讨小鼠附睾精子在显微注射进入卵母后的受精能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮在卵母卵黄形成期，它的RNA和DNA的合成均较快，因认为滤泡上皮合成分泌了一些物质参与卵黄蛋白的形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的与卵母电融合时可通过增加PVS中的数目来提高融合效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母又分裂成两个大小不对等的新：一个相对很大的卵子（成熟卵）和一个微小的次级极。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建受精的方法，并探讨小鼠附睾精子在注射进入卵母细胞后的受精能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮细胞在卵母细胞卵黄形成期，它的RNA和DNA的合成均较快，因认为滤泡上皮细胞合成分泌了一些物质参与卵黄蛋白的形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体细胞与卵母细胞电融合时可通过增加PVS中体细胞的数目融合效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母细胞又分裂成两个大小不对等的新细胞：一个相对很大的卵子（成熟卵细胞）和一个小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子显微注射进入细胞后的受精能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮细胞细胞黄形成期，它的RNA和DNA的合成均较快，因认为滤泡上皮细胞合成分泌了一些物质黄蛋白的形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体细胞细胞电融合时可通过增加PVS中体细胞的数目来提高融合效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级细胞又分裂成两个大小不对等的新细胞：一个相对很大的子（成熟细胞）和一个微小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子在显微注射进入母后的受精能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮在母黄形成期，它的RNA和DNA的合成均较快，因认为滤泡上皮合成分泌了一参与黄蛋白的形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体与母电融合时可通过增加PVS中体的数目来提高融合效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级母又分裂成两个大小不对等的新：一个相对很大的子（成熟）和一个微小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子在显微注射进入卵母细胞后的受精能力。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮细胞在卵母细胞卵黄形成期，它的RNA和DNA的合成均较，滤泡上皮细胞合成分泌了一些物质参与卵黄蛋白的形成。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体细胞与卵母细胞电融合时可通过增加PVS中体细胞的数目来提高融合效率。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母细胞又分裂成两个大小不对等的新细胞：一个相对很大的卵子（成熟卵细胞）和一个微小的次级极体。