This method is a better technique to synthesiz THDB, its inhibitory activity against tyrosinase is better than arbutin or kojic acid, equivalent to phloretin.
In the second step, the L-dopa formed by tyrosine hydroxylation is quickly decarboxylated by another enzyme called aromatic L-amino acid decarboxylase (AADC), to the neurotransmitter dopamine.
Aldolase A is made of 350 amino acids, the building blocks of proteins, but the bit that does the work consists of only three of these: lysine, glutamic acid and tyrosine. The rest of the enzyme is packaging.