Throw everything in the thermal cycler, wave your magic wand, and the process begins.

把所有成分加入热循环仪，挥动" 魔杖" ，这个过程就开始了。

So first of all, we're going to need a machine called a thermal cycler - that's where the PCR magic happens.

所以首先，我们需要一个叫做热循环仪的仪器，这是PCR发生的地方。

And the fact that it was always on reduced the thermal cycling of the filament and components limiting the stress caused by thermal expansion and contraction.

而且它始终于开启状态可减少灯丝和组件的热循环，从而限制由热膨胀和收缩引起的应力。

36. Question: In PCBA, what does the term " solder joint fatigue" mean? Answer: It refers to the weakening and eventual failure of solder joints due to repeated thermal cycling or mechanical stress.

36. 问： PCBA ，术语“焊点疲劳”是什么意思？答：是指由于反复的热循环或机械应力，焊点变弱并最终失效。

It requires a thermal cycler, filled with the DNA sample to be multiplied, thermostable Taq polymerase, primers with which we select what gets multiplied, and free nucleotides of all 4 types - A, T, C and G.

它需要往热循环仪加入用于扩增的DNA样本、耐热的Taq聚合酶、扩增时选用的引物，以及所有4种类型的游离核苷酸A、T、C和G。