Objective:HPLC was used to determine the contents of glycyrrhizic acid,isoliquiritin and isoliquiritigenin in compound liquorice tablets quantitatively.
A method for simultaneous separation and determination of two coumarins,dicumarol and cyclocoumarol,in cosmetics by HPLC-diode-array detector(DAD) was developed.
So the absorbing wavelength is selected,the operating condition optimized,and the quantitative and qualitative determination method on Polyoxin B by HPLC-DAD established.
Radix Rehmanniae, Cacumen Platycladi, Folium Artemisiae Argyi, Folium Nelumbinis were identified by TLC, and the content of quercitrin was determined by HPLC.
The results showed that the relative deviation of this method was less than 5% when compared with that of high-performance liquid chromatography, implying high accuracy of the presented biosensor in glucose detection in plants.