A method for simultaneous separation and determination of two coumarins,dicumarol and cyclocoumarol,in cosmetics by HPLC-diode-array detector(DAD) was developed.
Objective:HPLC was used to determine the contents of glycyrrhizic acid,isoliquiritin and isoliquiritigenin in compound liquorice tablets quantitatively.
The robot shows high mechanical strength, high load capacity and rapid locomotion speed in its solid phase as well as excellent shape adaptability in its liquid phase such as elongation, splitting and merging.
The results showed that the relative deviation of this method was less than 5% when compared with that of high-performance liquid chromatography, implying high accuracy of the presented biosensor in glucose detection in plants.