Any blood vessels encountered along the way are ligated.

操作过程中遇到的任何血管都要。

Open, identify, ligate, remove, irrigate, close.

打开，确认，，移出，冲洗，缝合。

Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功的细菌的筛选。

They found that it wasn't necessary to ligate a dog's pancreatic ducts in order to collect insulin.

他们发现没有必要狗的胰管来收集胰岛素。

It read, " Ligate pancreatic ducts of dogs." Banting's idea was to close off the small passages in a dog's pancreas that release its products into the rest of its body.

上面写：“狗的胰管。”班廷的想法是关闭狗胰腺中的小通道，这些通道将其产物释放到身体的其他部位。