Some are exchanged via small loops of DNA called plasmids.

一些基因会通过被称为质粒的DNA小环交换。

Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

除了包在拟核中的遗传物质外，许多细有额外的DNA分子，称为质粒。

However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

不过质粒通常包赋予细选择性优势的基因，例如抗生素耐药性。

Then they dropped the plasmid into a living bacterium, or yeast.

然后他们将质粒放入活细或酵母中。

Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

质粒所的基因相对较少，并且它们的基因对细说并不是必须的，缺少质粒的细胞往往也可以正常生长。

The plasmid enters and multiplies itself within a cell and can then be transferred to others.

质粒进入细胞并在细胞内繁殖， 然后可以转移到其他细胞中。

So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此本质上，些质粒是在不同细物之间转移抗生素抗性基因的载体。

This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

质粒突变以前在其他地方也见过；在中国，在动物和一些人类患者中。

Purification of the desired target fragment.

5。将片段与新的质粒载体连接组建一个新的重组分子。

This one had cell culture and transfections, antibodies, plasmids, recombinant virus production and infection, and so on and so forth.

个有细胞培养和转染、抗体、质粒、重组病毒生产和感染等等。

Initially, production in bacteria was the only route available and cDNA clones were used as they contained no introns.

cDNA需要与原核生物的转录和翻译信号相连，并且需要插入多拷贝的质粒。

But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

但是第一次在美国发现质粒，它可能会很快传播开。

Soon after, others began using plasmid vectors and adding genes for antibiotic resistance, greatly increasing the reach of the recombinant techniques. [81]

不久之后，其他人开始使用质粒载体并添加抗生素抗性基因，大大增加了重组技术的应用范围[81]。

Phage h can be used to clone fragments larger than plasmid vectors, and phage M13 allows cloned DNA to be isolated in single-stranded form.

更多载体被改造能同时拥有质粒和噬体的一些特性，像被称为粘粒的质粒-噬体λ杂交体。

More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

质粒和噬体已经在很多类的细中被使用表达基因，并且一些噬体也许已经被用将DNA整合到宿主基因组上。

Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

或者，用简单的英语说，质粒是有一天抗生素可能无法再帮助您治疗链球性咽喉炎的部分原因。

I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

实际上，所有些实验都是我亲手做的，当时我 58 岁，我还在培养质粒和喂养细胞，所以是非常不可能的。

The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

细胞质中有核糖体，它们是蛋白质合成的部位；拟核有遗传物质以及质粒；质粒有赋予细选择性优势的基因。

But also, a much more terrifying explanation has to do with plasmids, which are parts of the bacterial DNA. The plasmid enters and multiplies itself within a cell and can then be transferred to others.

而且，一个更可怕的解释与质粒有关，质粒是细 DNA 的一部分。 质粒进入细胞并在细胞内自我繁殖， 然后可以转移到其他细胞中。

This might be used to investigate a short region of a large cloned fragment in more detail, or to transfer a gene to a vector designed to express it in a particular species, for example.

以大肠杆中的质粒载体为例（最常见），个过程可分为以下步骤。