质粒

1.Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

果球孢链霉菌发酵液与溶菌酶均成功提取到粒。

2.NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.

以人高分化鼻咽癌细胞株(CNE1)为对象，采用电穿孔基因转染技术，将重组EBV-LMP表达粒转染CNE1细胞。

3.Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

方法利用球孢链霉菌发酵产生的变溶菌素来消化双歧杆菌细胞壁，提取粒，并与商业化溶菌酶法比较。