To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性酸脱氢酶特异地存在于鸟类和哺类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成臜化合物。颜形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因和促生长激素神经可以作为LDH患者疼痛神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性脱氢酶特异地存在于鸟类和哺类动物成熟睾丸和精子中，为精子运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成量与裂解细胞数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检活性，原位末端标记(TUNEL)方法检凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培清中,可用酶联检法30分钟量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合。颜色形成的量与裂解的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶检测法30钟测量。这种酶导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶检测法30钟测量。这种酶导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿法硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于哺乳动物的成熟睾丸精子中，为精子的运动存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

应MTT、LDH检测细胞活性，原位末端标(TUNEL)法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清,酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。

To detect cytoactive by MTT and LDH, and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling (TUNEL).

用MTT、LDH检测细胞活性，原位末端标记(TUNEL)方法检测细胞凋亡。

Conclusions. These findings indicate the role of plasma CGRP and possibly galanin as a systemic neurochemical signature of pain states in patients with LDH.

降钙素基因相关肽和促生长激素神经肽可以作为LDH患者疼痛的神经化学指标。

Objective To observe the near future curative effect and that of the distant future on LDH with the treatment of naprapathy six methods and epidrual administration of drugs.

目的观察推拿六法加硬膜外给药治疗陈旧性腰椎间盘突出症(LDH)的近远期效果并讨其治疗机理。

LDH-C4 is specific to mature testes and spermtozoa of bird and mammal.It is related to metabolic processes that provide energy for motility and survival of spermtozoa.LDH-C4 is an autoantigen.

摘要精子特异性乳酸脱氢酶特异地存在于鸟类和哺乳类动物的成熟睾丸和精子中，为精子的运动和存活提供能量。

Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放出的乳酸脱氢酶存在于培养物上清中,可用酶检测法30钟测量。这种酶导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。

In the group of dicoumarol plus doxorubicin, serum ALT, LDH, CK activities were significantly increased as compared with that of doxorubicin group alone, and liver MDA content was also increased.

与单给阿霉素组比较，双香豆素能显著增强阿霉素所致的血清ALT、LDH、CK催化活性升高，肝MDA含量也增高。